Designing PCR and Sanger Sequencing Primers– Seq It Out #5
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thermofisher.com
Designing PCR and Sanger Sequencing Primers– Seq It Out #5
Primer design is like art. There is more than one design to cover the region of interest. Are you an “Artist”? Primers are crucial to the success of target amplification and subsequent sequencing in PCR and Sanger sequencing workflows. Let’s take a look at our lab book: In the typical Sanger sequencing workflow from genomic
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